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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/12/2011 |
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Data da última atualização: |
03/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOODWIN, S. B.; M’BAREK, S. B.; DHILLON, B.; WITTENBERG, A. H. J.; CRANE, C. F.; HANE, J. K.; FOSTER, A. J.; LEE, T. A. J. van der; GRIMWOOD, J.; AERTS, A.; ANTONIW, J.; BAILEY, A.; BLUHM, B.; BOWLER, J.; BRISTOW, J.; BURGT, A. van der; CANTO CANCHE, B.; CHURCHILL, A. C. L.; CONDE FERRÀEZ, L.; COOLS, H. J.; COUTINHO, P. M.; CSUKAI, M.; DEHAL, P.; WIT, P. de; DONZELLI, B.; GEEST, H. C. van de; HAM, R. C. H. H. van; HAMMOND KOSACK, K. E.; HENRISSAT, B.; KILIAN, A.; KOBAYASHI, A. K.; KOOPMANN, E.; KOURMPETIS, Y.; KUZNIAR, A.; LINDQUIST, E.; LOMBARD, V.; MALIEPAARD, C.; MARTINS, N. F.; MEHRABI, R.; NAP, J. P. H.; PONOMARENKO, A.; RUDD, J. J.; SALAMOV, A.; SCHMUTZ, J.; SCHOUTEN, H. J.; SHAPIRO, H.; STERGIOPOULOS, I.; TORRIANI, S. F. F.; TU, H.; VRIES, R. P. de; WAALWIJK, C.; WARE, S. B.; WIEBENGA, A.; ZWIERS, L.; OLIVER, R. P.; GRIGORIEV, I. V.; KEMA, G. H. J. |
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Afiliação: |
STEPHEN B. GOODWIN, USDA–AGRICULTURAL RESEARCH SERVICE; SARRAH BEN M’BAREK, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; BRAHAM DHILLON, PURDUE UNIVERSITY, USA; ALEXANDER H. J. WITTENBERG, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; CHARLES F. CRANE, USDA–AGRICULTURAL RESEARCH SERVICE; JAMES K. HANE, MURDOCH UNIVERSITY, PERTH, AUSTRALIA; ANDREW J. FOSTER, IBWF e.V., GERMANY; THEO A. J. VAN DER LEE, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; JANE GRIMWOOD, HUDSONALPHA INSTITUTE OF BIOTECHNOLOGY, USA; ANDREA AERTS, DOE JOINT GENOME INSTITUTE, USA; JOHN ANTONIW, ROTHAMSTED RESEARCH, UNITED KINGDOM; ANDY BAILEY, UNIVERSITY OF BRISTOL, UNITED KINGDOM; BURT BLUHM, UNIVERSITY OF ARKANSAS, USA; JUDITH BOWLER, SYNGENTA, UNITED KINGDOM; JIM BRISTOW, HUDSONALPHA INSTITUTE OF BIOTECHNOLOGY, USA; ATE VAN DER BURGT, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; BLONDY CANTO CANCHE, CENTRO DE INVESTIGACIÓN CIENTÍFICA DE YUCATÁN, MÉXICO; ALICE C. L. CHURCHILL, CORNELL UNIVERSITY, USA; LAURA CONDE FERRÀEZ, CENTRO DE INVESTIGACIÓN CIENTÍFICA DE YUCATÁN, MÉXICO; HANS J. COOLS, ROTHAMSTED RESEARCH, UNITED KINGDOM; PEDRO M. COUTINHO, ARCHITECTURE ET FONCTION DES MACROMOLECULES BIOLOGIQUES, CNRS, FRANCE; MICHAEL CSUKAI, SYNGENTA, UNITED KINGDOM; PARAMVIR DEHAL, DOE JOINT GENOME INSTITUTE, USA; PIERRE DE WIT, WAGENINGEN UNIVERSITY AND RESEARCH CENTRE, THE NETHERLANDS; BRUNO DONZELLI, USDA–AGRICULTURAL RESEARCH SERVICE; HENRI C. VAN DE GEEST, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; ROELAND C. H. J. VAN HAM, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; KIM E. HAMMOND KOSACK, ROTHAMSTED RESEARCH, UNITED KINGDOM; BERNARD HENRISSAT, ARCHITECTURE ET FONCTION DES MACROMOLECULES BIOLOGIQUES, CNRS, FRANCE; ANDRZEJ KILIAN, DIVERSITY ARRAYS TECHNOLOGY PTY LTD, AUSTRALIA; ADILSON KENJI KOBAYASHI, CPAMN; EDDA KOOPMANN, BAYER CROPSCIENCE AG, GERMANY; YIANNIS KOURMPETIS, WAGENINGEN UNIVERSITY AND RESEARCH CENTRE, THE NETHERLANDS; ARNOLD KUZNIAR, WAGENINGEN UNIVERSITY AND RESEARCH CENTRE, THE NETHERLANDS; ERIKA LINDQUIST, DOE JOINT GENOME INSTITUTE, USA; VINCENT LOMBARD, ARCHITECTURE ET FONCTION DES MACROMOLECULES BIOLOGIQUES, CNRS, FRANCE; CHRIS MALIEPAARD, WAGENINGEN UNIVERSITY AND RESEARCH CENTRE, THE NETHERLANDS; NATALIA FLORENCIO MARTINS, CENARGEN; RAHIM MEHRABI, SEED AND PLANT IMPROVEMENT INSTITUTE, IRAN; JAN P. H. NAP, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; ALISA PONOMARENKO, PURDUE UNIVERSITY, USA; JASON J. RUDD, ROTHAMSTED RESEARCH, UNITED KINGDOM; ASAF SALAMOV, DOE JOINT GENOME INSTITUTE, USA; JEREMY SCHMUTZ, HUDSONALPHA INSTITUTE OF BIOTECHNOLOGY, USA; HENK J. SCHOUTEN, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; HARRIS SHAPIRO, DOE JOINT GENOME INSTITUTE, USA; IOANNIS STERGIOPOULOS, WAGENINGEN UNIVERSITY AND RESEARCH CENTRE, THE NETHERLANDS; STEFANO F. F. TORRIANI, SWISS FEDERAL INSTITUTE OF TECHNOLOGY (ETH), SWITZERLAND; HANK TU, DOE JOINT GENOME INSTITUTE, USA; RONALD P. DE VRIES, CBS–KNAW FUNGAL BIODIVERSITY CENTRE, THE NETHERLANDS; CEES WAALWIJK, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; SARAH B. WARE, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS; AD WIEBENGA, CBS–KNAW FUNGAL BIODIVERSITY CENTRE, THE NETHERLANDS; LUTE-HARM ZWIERS, CBS–KNAW FUNGAL BIODIVERSITY CENTRE, THE NETHERLANDS; RICHARD P. OLIVER, CURTIN UNIVERSITY, AUSTRALIA; IGOR V. GRIGORIEV, DOE JOINT GENOME INSTITUTE, USA; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL B.V., WAGENINGEN, THE NETHERLANDS. |
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Título: |
Finished genome of the fungal wheat pathogen Mycosphaerella graminicola Reveals dispensome structure, chromosome plasticity, and stealth pathogenesis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Plos Genetics, v. 7, n. 6, e1002070, 2011. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Fungus. |
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Thesagro: |
Fungo. |
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Thesaurus Nal: |
Mycosphaerella graminicola. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53161/1/AdilsonKobayashi.pdf
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Marc: |
LEADER 02207naa a2200817 a 4500
001 1913905
005 2022-06-03
008 2011 bl uuuu u00u1 u #d
100 1 $aGOODWIN, S. B.
245 $aFinished genome of the fungal wheat pathogen Mycosphaerella graminicola Reveals dispensome structure, chromosome plasticity, and stealth pathogenesis.$h[electronic resource]
260 $c2011
650 $aMycosphaerella graminicola
650 $aFungo
653 $aFungus
700 1 $aM’BAREK, S. B.
700 1 $aDHILLON, B.
700 1 $aWITTENBERG, A. H. J.
700 1 $aCRANE, C. F.
700 1 $aHANE, J. K.
700 1 $aFOSTER, A. J.
700 1 $aLEE, T. A. J. van der
700 1 $aGRIMWOOD, J.
700 1 $aAERTS, A.
700 1 $aANTONIW, J.
700 1 $aBAILEY, A.
700 1 $aBLUHM, B.
700 1 $aBOWLER, J.
700 1 $aBRISTOW, J.
700 1 $aBURGT, A. van der
700 1 $aCANTO CANCHE, B.
700 1 $aCHURCHILL, A. C. L.
700 1 $aCONDE FERRÀEZ, L.
700 1 $aCOOLS, H. J.
700 1 $aCOUTINHO, P. M.
700 1 $aCSUKAI, M.
700 1 $aDEHAL, P.
700 1 $aWIT, P. de
700 1 $aDONZELLI, B.
700 1 $aGEEST, H. C. van de
700 1 $aHAM, R. C. H. H. van
700 1 $aHAMMOND KOSACK, K. E.
700 1 $aHENRISSAT, B.
700 1 $aKILIAN, A.
700 1 $aKOBAYASHI, A. K.
700 1 $aKOOPMANN, E.
700 1 $aKOURMPETIS, Y.
700 1 $aKUZNIAR, A.
700 1 $aLINDQUIST, E.
700 1 $aLOMBARD, V.
700 1 $aMALIEPAARD, C.
700 1 $aMARTINS, N. F.
700 1 $aMEHRABI, R.
700 1 $aNAP, J. P. H.
700 1 $aPONOMARENKO, A.
700 1 $aRUDD, J. J.
700 1 $aSALAMOV, A.
700 1 $aSCHMUTZ, J.
700 1 $aSCHOUTEN, H. J.
700 1 $aSHAPIRO, H.
700 1 $aSTERGIOPOULOS, I.
700 1 $aTORRIANI, S. F. F.
700 1 $aTU, H.
700 1 $aVRIES, R. P. de
700 1 $aWAALWIJK, C.
700 1 $aWARE, S. B.
700 1 $aWIEBENGA, A.
700 1 $aZWIERS, L.
700 1 $aOLIVER, R. P.
700 1 $aGRIGORIEV, I. V.
700 1 $aKEMA, G. H. J.
773 $tPlos Genetics$gv. 7, n. 6, e1002070, 2011.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
19/01/2009 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
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Afiliação: |
ADILSON KENJI KOBAYASHI, CPAMN; CAUCASELLA DIAZ TRUJILLO, PLANT RESEARCH INTERNATIONAL; THEO VAN DER LEE; LUTE-HARM ZWIERS; LUCIANO V. PAIVA, FEDERAL UNIVERSITY OF LAVRAS; MANOEL TEIXEIRA SOUZA JUNIOR, EMBRAPA LABEX EUROPE; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL. |
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Título: |
Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana. MenosMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducte... Mostrar Tudo |
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Palavras-Chave: |
CIRAD139A; CIRAD86; DsRed; GFP. |
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Thesagro: |
Doença; Fungo. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/69938/1/GenerationTransgenic-22676.pdf
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Marc: |
LEADER 02408nam a2200253 a 4500
001 1069938
005 2023-07-24
008 2008 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, A. K.
245 $aGeneration of transgenic Mycosphaerella fijiensis expressing reporter genes$btools for pathogenicity and mating studies.$h[electronic resource]
260 $aIn: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland$c2008
520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana.
650 $aDoença
650 $aFungo
653 $aCIRAD139A
653 $aCIRAD86
653 $aDsRed
653 $aGFP
700 1 $aDIAZ TRUJILLO, C.
700 1 $aLEE, T. van der
700 1 $aZWIERS, L.-H.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aKEMA, G. H. J.
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